Journal: bioRxiv

Article Title: Distinct regulation of dopamine D3 receptor in the basolateral amygdala and dentate gyrus during the reinstatement of cocaine CPP induced by drug priming and social stress

doi: 10.1101/2021.01.08.425788

Figure Lengend Snippet: Mice were conditioned to cocaine and after 60 days of abstinence they were administered with saline (veh+sal; controls), with a dose of cocaine prime (veh+coc) or with an injection of the D3DR antagonist SB-277011-A (24 mg/kg i.p.) prior to the cocaine-priming (SB24+coc). A preference test was conducted giving the animals free access to both compartments of the CPP apparatus for 15 min. Mice were sacrificed immediately after the reinst test. + p<0.05 vs veh+coc. No significant changes in D3DR or DAT levels were found between groups in dentate gyrus. Each bar corresponds to mean ± standard error of the mean.

Article Snippet: Briefly, the conditioned place preference apparatus (CPP; Panlab, Barcelona, Spain) utilized to induce a reliable preference is based on that used by Valverde et al. (1996) [ ] and consists in a box with two equally sized chambers (20 L × 18 W × 25 H cm) interconnected by a rectangular corridor (20L x 7W x 25H cm).

Techniques: Injection

Journal: bioRxiv

Article Title: Distinct regulation of dopamine D3 receptor in the basolateral amygdala and dentate gyrus during the reinstatement of cocaine CPP induced by drug priming and social stress

doi: 10.1101/2021.01.08.425788

Figure Lengend Snippet: Mice were conditioned to cocaine and after 60 days of abstinence they were injected with vehicle and exposed to a non-defeated agonistic encounter (veh+ND; controls) or exposed to an agonistic encounter with social defeat (veh+SD). Also, a group of animals was injected with the D3DR antagonist SB-277011-A (12 or 24 mg/kg i.p.) prior to the social-defeated agonistic encounter (SB12+SD and SB24+SD, respectively). A preference test was conducted giving the animals free access to both compartments of the CPP apparatus for 15 min. Mice were sacrificed immediately after the reinst test. *p<0.05 vs veh+ND; + p<0.05, ++ p<0.01 vs veh+SD. No significant changes in D3DR or DAT levels were found between groups in dentate gyrus. Each bar corresponds to mean ± standard error of the mean.

Article Snippet: Briefly, the conditioned place preference apparatus (CPP; Panlab, Barcelona, Spain) utilized to induce a reliable preference is based on that used by Valverde et al. (1996) [ ] and consists in a box with two equally sized chambers (20 L × 18 W × 25 H cm) interconnected by a rectangular corridor (20L x 7W x 25H cm).

Techniques: Injection

Journal: bioRxiv

Article Title: Distinct regulation of dopamine D3 receptor in the basolateral amygdala and dentate gyrus during the reinstatement of cocaine CPP induced by drug priming and social stress

doi: 10.1101/2021.01.08.425788

Figure Lengend Snippet: All the animals were conditioned to cocaine preference and underwent through abstinence for 60 days. (A) and (B) show the cohort of animals that were administered with saline (veh+sal; controls), with a dose of cocaine prime (veh+coc) or with an injection of the D3DR antagonist SB-277011-A (24 mg/kg i.p.) prior to the cocaine-priming (SB24+coc). *p<0.05, **p<0.01 vs veh+sal. (C) and (D) represent the set of animals that were injected with vehicle and exposed to a non-defeated agonistic encounter (veh+ND; controls) or exposed to an agonistic encounter with social defeat (veh+SD). Also, a group of animals was injected with the D3DR antagonist SB-277011-A (12 or 24 mg/kg i.p.) prior to the social-defeated agonistic encounter (SB12+SD and SB24+SD, respectively). A preference test was conducted by giving free access to both compartments of the CPP apparatus for 15 min to every group of animals. *p<0.05, **p<0.01 vs veh+ND; + p<0.05 vs veh+SD. Each bar corresponds to mean ± standard error of the mean.

Article Snippet: Briefly, the conditioned place preference apparatus (CPP; Panlab, Barcelona, Spain) utilized to induce a reliable preference is based on that used by Valverde et al. (1996) [ ] and consists in a box with two equally sized chambers (20 L × 18 W × 25 H cm) interconnected by a rectangular corridor (20L x 7W x 25H cm).

Techniques: Injection